针刺足三阳经穴位对家兔胃黏膜一氧化氮及一氧化氮合酶的影响

Effect of acupuncture at the channel of foot-sanyang on nitrogen monoxide and nitricoxide synthase in gastric mucosa of rabbits

目的:比较针刺足三阳经穴位对家兔胃黏膜损伤保护作用,探讨经脉-脏腑相关的特异性。方法:以新西兰白兔为研究对象,分空白组、模型组、胃经组、胆经组及膀胱经组,用无水乙醇灌胃制造急性胃黏膜损伤模型,以胃黏膜损伤指数、一氧化氮及一氧化氮合酶(nitrousoxidesynthetase,NOS)含量变化为观察指标,比较针刺治疗后各指标差异。结果:①胃黏膜损伤指数:胃经组(10.88±3.23)明显低于模型组(24.88±6.29)、胆经组(19.38±3.66)及膀胱经组(24.13±1.64)。②胃黏膜一氧化氮含量(μmol/g):胃经组(1.38±0.27)明显高于模型组(0.45±0.20)、胆经组(0.45±0.19)及膀胱经组(0.57±0.24),差异有显著性意义(F=11.32,P<0.01)。③胃黏膜NOS含量(mkat/g):胃经组(32.20±10.21)明显高于模型组(12.70±2.61)、胆经组(22.12±5.32)及膀胱经组(12.52±4.24);而胆经组又明显高于模型组及膀胱经组,差异有显著性意义(F=17.664,P<0.01)。结论:①针刺足阳明经穴可升高实验兔胃黏膜一氧化氮、NOS含量,减低胃黏膜损伤指数,表现出良好的胃黏膜保护作用。②针刺足少阳经穴可升高实验兔胃黏膜NOS含量,但作用较足阳明经穴组弱。③足阳明经与胃具有特异相关性。

AIM:To compare the protective effect on gastric mucosal lesion of rabbits by u sing acupuncture to the channel of foot-sanyang,and to explore the specific cor relation between meridians and viscera. METHODS:New Zealand rabbits were divided into five groups:blank group,model gr oup,stomach channel group,gallbladder channel group and bladder channel group.Mo dels of acute gastric mucosal lesion were established by injection of pure ethyl alcohol into the stomach of rabbits.The index of gastric mucosal lesion and the changes of the levels of nitrogen monoxide(NO) and nitricoxide synthase(NOS) we re the indexes of observation,and the differences were compared after treatment of acupuncture. RESULTS:①The index of gastric mucosal lesion in the stomach channel group(10. 88±3.23) was significantly lower than those in the model group(24.88±6.29),gal lbladder channel group(19.38±3.66) and bladder channel group(24.13±1.64).②The level of NO(μmol/g) in gastric mucosa in the stomach channel group(1.38±0.27) was apparently higher than those in the model group(0.45±0.20),gallbladder cha nnel group(0.45±0.19) and bladder channel group(0.57±0.24)(F=11.32,P< 0.01).③ The level of NOS(mkat/g) in gastric mucosa in the stomach channel group(32.20±1 0.21) was markedly higher than those of the model group(12.70±2.61),gallbladder channel group(22.12±5.32) and bladder channel group(12.52±4.24),while that in the gallbladder channel group was obviously higher than those in the model grou p and bladder channel group(F=17.664,P< 0.01). CONCLUSION:①Acupuncture at the stomach channel of foot-yangming can increase the levels of NO and NOS in gastric mucosa and decrease the indexes of gastric mucosal lesion,which suggests that acupuncture has an excellent protective effec t on lesion of gastric mucosa.②Acupuncture at the gallbladder channel of foot- shaoyang can increase the level of NOS in gastric mucosa,however,the effect is w eaker compared with the channel of foot-yangming.③There is a specific correlat ion between stomach channel of foot-yangming and stomach.