普萘洛尔对去卵巢大鼠骨密度及血清白细胞介素6的影响(英文)

Effects of propranolol to bone density and serum interleukin-6 in ovariectomized rats

背景:交感神经系统影响骨代谢的具体作用和机制不明确。目的:探讨普萘洛尔对去卵巢大鼠骨密度及对血清白细胞介素6(IL-6)水平的影响,研究β受体阻滞剂对骨质疏松的防治作用及机制。设计:随机对照的实验研究。地点、材料和干预:本实验在中国中医研究院基础所完成。将50只健康雌性未交配6个月龄SD大鼠随机分为5组:①假手术组。②去卵巢(OVX)后雌激素治疗(O+E)组。③OVX后普萘洛尔治疗(O+P)组。④OVX后雌激素+普萘洛尔治疗(O+E+P)组。⑤OVX后安慰剂(OVX)组。OVX后1周开始经灌胃给予普萘洛尔、皮下注射17β-雌二醇,共12周。动物处死时留取第3～5腰椎、血清标本,进行骨密度分析及血清IL-6水平测定。主要观察指标:各组大鼠椎骨骨密度及血清瘦素水平。结果:O+P,O+P+E,O+E组的骨密度较OVX组明显增高(P<0.05),血清IL-6水平OVX组犤(336.6±18.1)ng/L犦较假手术组犤(208.8±17.2)ng/L犦明显增高(t=16.982,P<0.01),O+E组犤(252.7±16.3)ng/L犦,O+P组犤(274.4±20.0)ng/L犦,O+P+E组犤(222.9±16.5)ng/L犦均明显低于OVX组犤(336.6±18.1)ng/L犦(t=11.160,7.783,14.971,P<0.01)。结论:普萘洛尔能使卵巢切除术后骨质疏松大鼠的骨密度改善,能有效地预防OVI大鼠的骨丢失,同时降低血清水平。

BACKGROUND:The mechanism how the sympathetic nervous system affects bone metab olism remains unclear. OBJECTIVE:To explore the effects of propranolol to bone density and serum inte rleukin-6 in ovariectomized rats and to study the preventive function and mecha nism of βreceptor blocking agent to osteoporosis. DESIGN:Randomized case-control trial study. SETTING,PARTICIPANTS and INTERVENTIONS:The study was done in Foundamental Rese arch Institution of Academy of Traditional Chinese Medicine.Fifty 6-month-old unmated female SD rats were randomly divided into 5 groups:sham operated group,o variectomized and treated with estrogen group(O+E),ovariectomized and treated w ith propranolol group(O+P),ovariectomized and treated with both estrogen and pr opranolol group(O+E+P),ovariectomized and treated with placebo group(OVX). Pro pranolol was infused to stomach and 17 β-estradiol was given subcutaneously fr om the first week after ovariectomy for 12 weeks.The 3-5 lumbar vertebras and b lood serum samples were collected to do the bone density analysis and assay of s erum interleukin-6(IL-6). MAIN OUTCOME MEASURES:Spinal bone density and the values of serum IL-6 in ea ch group. RESULTS:The bone densities in the O+P,O+P+E and O+E groups were much high er than that of the OVX(P< 0.05),the serum IL-6 in the OVX group[(336.6±18.1) ng/L] was higher than that in the sham operated group[(208.8±17.2) ng/L](t=16.9 82,P< 0.01) and was also higher than those in the O+E[(252.7±16.3) ng/L],O+P[ (274.4±20.0)ng/L] and O+P+E[(222.9±16.5)ng/L] groups(t=11.160,7.783,14.971,P < 0.01). CONCLUSION:Propranolol could improve the bone density in ovariectomized rats suffered from osteoporosis and efficiently prevent the bone loss and reduce the level of serum IL-6 simultaneously.