猪主动脉瓣去细胞的初步研究

The study of decellularized porcine heart valve for Tissue engineering heart valve

目的 对猪主动脉瓣进行了酶水化法去细胞的研究,探讨应用猪去细胞瓣膜支架体外构建组织工程心脏瓣膜的可行性。方法 应用胰蛋白酶/乙二胺四乙酸去除猪主动脉瓣组织中的细胞,对处理后的组织进行组织学与免疫组化,扫描电子显微镜观察,并进行理化性能测定。结果 酶水化法去细胞效果良好,且能较好地保持胶原纤维和弹性纤维原有的排列和分布。与新鲜猪主动脉瓣比较,热皱缩温度、抗张强度差异无统计学意义(P>0.05)。结论 酶水化法去细胞效果良好,初步制造了猪主动脉瓣脱细胞基质材料,为进一步制作组织工程瓣膜提供了较合适的支架材料。

Objective To obtain the decellularized scaffold for tissue engineering heart valvefrom porcine heart valves.Methods Trypsin and EDTA were used to decellularize fresh porcine aorticvalves. The morphological integrity of the acellular scaffold was investigated by means of light andscanning electron microscopy. The shrinkage temperature, tension and strain at fracture were comparedbetween fresh porcine aortic valves and acellular matrix.Results Light and electron microscopyconfirmed that all the cellular constituents were removed without ultrastructural damage to fibrouscomponents. Compared with the fresh sample, there were no significant difference [P>0.05] at theevaluation of Shrinkage temperature, tension at fracture and strain at fracture.Conclusion Porcineaortic valves can be almost completely acellularized by trypsin/EDTA procedure without impairing themechanical property of the tissue. This kind of acellular tissue could possibly be used to develop tissueengineering for heart valve substitute.