基质金属蛋白酶MMP-9及其组织抑制剂TIMP-1在卵巢肿瘤中的表达及意义

Messenger RNA expression of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinase -1 in ovarian tumors

目的 研究基质金属蛋白酶MMP - 9及组织抑制剂TIMP - 1在卵巢肿瘤中的mRNA表达及与卵巢癌临床病理特征和预后的关系。方法 应用RT -PCR方法检测 4 8例卵巢癌、 2 1例良性卵巢肿瘤及 2 2例正常卵巢组织中MMP - 9及TIMP - 1mRNA的表达情况 ,进行阳性率及半定量的比较 ,并将其结果与临床及病理学资料进行统计学分析。结果 MMP - 9在卵巢癌中表达的阳性率及半定量明显高于正常卵巢组织 ,其表达水平与卵巢癌的手术病理分期及预后有关 ,在Ⅲ -Ⅳ期患者中的表达显著高于Ⅰ -Ⅱ期患者 (P <0 0 5 )。MMP - 9阳性患者累积生存率明显低于阴性者。TIMP - 1的表达在卵巢癌中无明显增高 ,且与临床病理无相关性。MMP - 9/TIMP- 1在卵巢癌中的比值明显高于正常卵巢组织。结论 MMP - 9及MMP - 9与TIMP - 1的平衡失调与卵巢癌的浸润转移密切相关 ,MMP - 9可作为监测晚期卵巢癌患者预后的一个指标。

Objective To investigate the mRNA expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1)and the relationship between the expression and clinic-pathological characteristics of patients with ovarian tumor.Methods RT-PCR was employed to assay the mRNA level of MMP-9 and TIMP-1 in 48 cases of ovarian cancer,21 benign ovarian tumor and 22 normal ovaries.Results The positive rate and semi-quantitative level of MMP-9 mRNA and the ratio of MMP-9/TIMP-1 were significantly higher in ovarian cancer than those in normal ovarian ( P <0 05)and the expression level of MMP-9 in patients with stage Ⅲ and Ⅳ was significantly higher than in stageⅠand Ⅱ ( P <0 05).The cumulate survival rate of patients with MMP-9 positive expression is lower than that with negative expression.Conclusions The over-expression of MMP-9 and the imbalance between MMP-9 and TIMP-1 show significant relevance to the invasion and metastasis of ovarian cancer.MMP-9 may act as a prognostic indicator in patients with ovarian cancer.