脾切除肝硬化门脉高压大鼠血浆TNF-α质量浓度的变化

Effect of Tumor Necrosis Factor on Venous Plasma of Portal Hypertension Rat Perserved Splen

为研究脾切除术、脾动脉结扎术对肝硬化门脉高压症 (PHT)大鼠血浆肿瘤坏死因子 (TNF α)的影响 ,研究PHT时TNF α与一氧化氮合酶 (NOS)间的关系 ,建成PHT大鼠模型 ,用放射免疫分析法动态检测TNF α ,根据吸光度的大小测定血浆NOS活性 ,并观察术后 4周内各组的病死率。发现 :PHT大鼠血浆TNF α质量浓度较正常对照组明显增高 (P <0 .0 1 ) ;PHT脾切除组术后 3d、7d、1 4d血浆TNF α质量浓度与实验对照组比较有显著性差异 (P <0 .0 1 ) ;脾动脉结扎组TNF α的质量浓度虽比实验对照组升高 ,但无显著性差异。实验对照组血浆NOS活性明显高于正常对照组 (P <0 .0 1 ) ,且与TNF α显著正相关 (P <0 .0 1 ,r =0 .946) ;脾动脉结扎组NOS活性显著低于实验对照组及脾切除组 (P <0 .0 1 )。PHT时血浆TNF α的质量浓度显著增高 ,提示 :TNF α可能在PHT形成中起重要作用。与脾动脉结扎组相比较 ,脾切除组血浆TNF α的质量浓度显著增高 ,血浆NOS活性以及病死率亦显著增高 ,提示 :脾动脉结扎术后脾脏仍可发挥一定作用 ,PHT时应保留脾脏。

The aim was to study the effect of portal hypertension (PHT) on tumor necrosis factor (TNF-α) in venous plasma, the influence of splenectomy and splenic artery ligation on TNF-α levels in venous plasma in model rats and to investigate the relation between TNF-α and NOS levels in venous plasma in PHT rats. Healthy Wistar rats were randomized into four groups: The latter three groups were used to established PHT model. TNF-α levels in venous plasma were dynamically measured by radioimmunoassay (RIA) at points after the splenectomy and SAL; NOS levels in venous plasma were determined 14 d after operation. The mortality between SLC group and SAL group were compared 4 weeks after operation. Light microscope were applied to observe the pathological change. PHT TNF-αlevels in venous plasma were significantly higher than NCG group (P<0.01). SLC group TNF-αin plasma increased significantly, compared with MCG group(P<0.01), at points (3 d, 7 d, and 14 d) after splenectomy. SAL group TNF-α levels in venous plasma were slightly higher than MCG group ( P> 0.05 ), and were significantly lower than SLC group ( P< 0.01 ) at points (3 d, 7 d, 14 d) after SAL. MCG group NOS levels in venous plasma were significantly elevated(P<0.01), compared with NCG group, there was a positive correlation(r=0.946, P<0.01) between TNF-α levels and NOS levels in venous plasma, while SAL group NOS levels were significantly reduced ( P< 0.01 ), compared with MCG group and SLC group respectively. The morbidity in SLC group was remarkably higher than that of SAL group ( P< 0.05 ). In PHT, TNF-α levels in venous plasma significantly increase, possibly play an important role in the course of PHT. TNF-αlevels in venous plasma significantly increase after splenectomy, while the morbidity also elevate remarkably. Compared with PHT model rats, SAL group TNF-α levels and the morbidity don't increas significantly, and prognosis can be improved.