期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

耐热碱性磷酸酯酶的研究进展 被引量：2

The Study of Thermostable Alkaline Phosphatase
下载PDF
导出
摘要 叙述耐热碱性磷酸酯酶 (TAP)的来源、性质、热稳定性及应用。分析影响TAP酶活的因素 ,通过对酶辅助因子的研究 ,探索酶的活性位点。介绍了提高耐热AP的酶比活及产量的途径。 The application and development of TAP is recounted. The thermostablility of TAP is investigated. The factors of influencing the activity of TAP are analyzed. With the study of cofactor, the active sites can be investigated. The approaches of increasing TAP's specific activity and yield are analyzed.
作者 龚宁萍 陈朝银
机构地区 昆明理工大学生物工程系
出处 《药物生物技术》 CAS CSCD 2004年第3期207-210,共4页 Pharmaceutical Biotechnology
基金 云南省应用基础研究基金项目资助 No .98C0 95M
关键词 耐热碱性磷酸酯酶 热稳定性 辅助因子 Thermostable alkaline phosphatase, Thermostablity, Cofactor
分类号 Q55 [生物学—生物化学]
  • 相关文献

参考文献17

  • 1Engvall E, Perlman P. Enzyme-liked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G[J]. Immunochemistry,1971,8:871.
  • 2Jablonski E, Moomaw E W, Tullis R H, etal. Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes[J]. Nucleic Acids Res,1986,14:6115.
  • 3季朝能,白晓阳,盛小禹,张冰,毛裕民.信号肽去除的耐热碱性磷酸酯酶FD-TAP在大肠杆菌中的亚克隆和高表达[J].生物工程学报,2000,16(6):690-694. 被引量：23
  • 4Cannio R, Rossi M, Bartolucci S. A few amino acid substitutions are responsible for the higher thermostability of a novel NAD(+)-dependent bacillar alcohol dehydrngenase[J]. Eur J Biochem, 1994,222:345.
  • 5Dealwis CG, Chen L, Brennan C, et al. 3-D structure of the D153G mutant of Escherichia coli alkaline phosphatase: an enzyme with weaker magnesium binding and increased catalytic activity[J]. Protein Eng, 1995, 8: 865.
  • 6Min-Fung Yeh, John M Trela. Purifucation and characterization of a repressible alkaline phosphatase from Thermus aquaticus[J]. J of Biol Chem, 1976,25:3134.
  • 7Dong G, Zeikus JG. Purification and characterization of alka line phosphatase from Thermotoga neapolitana[J]. Enzyme Microb Technol , 1997, 21:335.
  • 8盛小禹,王曦,高静波,毛裕民.嗜热细菌的碱性磷酸酯酶的研究[J].生物化学杂志,1997,13(6):672-676. 被引量：17
  • 9Sebastien zappa, Jean-Luc R, Didier F, et al. Characterization of a highly thermostable alkaline phosphatase from the Euryarchaeon Pyrococcusabyssi[J].Appl And Environ Microbiol, 2001,67:4504.
  • 10季朝能,盛小禹,白晓阳,姜涛,毛裕民.耐热碱性磷酸酯酶(TAPND27)的表达、纯化和性质测定[J].复旦学报（自然科学版）,2000,39(3):297-301. 被引量：6

二级参考文献12

共引文献41

同被引文献47

  • 1杨安中,牟筱玲,李孟良,余海兵.喷施细胞分裂素类物质对地膜旱作水稻防衰及增产效应[J].水土保持学报,2005,19(2):199-200. 被引量：19
  • 2郭彦,张文会,魏秀俭,赵深.6-BA对干旱胁迫条件下水稻芽期生长的影响[J].安徽农业科学,2005,33(9):1592-1593. 被引量：2
  • 3蔡永萍,陈静娴,聂凡.水稻抽穗期喷施6—BA,ABA对旗叶衰老及同化物运输的影响[J].安徽农业科学,1996,24(2):113-115. 被引量：5
  • 4Chen,L.C.,et al.1997.Extracellular proteins of Cryptococcus neoformans and host antibody response,Infect Immun.65:2 599-2 605
  • 5Gloria,S.,et al.2002.Systematics of basidiomycetous yeasts:A comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions.FEMS Yeast Research,2:495-517
  • 6John,G.H.et al.1994.Manual of Determinative Bacteriology.Ninth Edition.Williams and Wilkins Press
  • 7Kurtzman,C.P.et al.1997.Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene.J.Clinical microbiology,35(5):1 216-1 223
  • 8Lowry,O.H.et al.1951.Protein measurement with the phenol folin reagent,J.Biol.Chem.193:265-275
  • 9Mentlien,R.et al.1984.Hydrolysis of ester and amide-type drugs by purified isoenzymes of non-specific carboxylseterase from rat,sheep and horse liver,Biochemical.Pharmacol,33:124-1248
  • 10Plow,F.J.et al.1996.High-yield production of mono-and di-oleylglycerol by lipase-catalysed hydrolysis of triolein,Enzyme Microb.Technol.18:66-71

引证文献2

二级引证文献7

药物生物技术
2004年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部