CD154胞外区基因局部修饰对大鼠移植肾存活的影响

Effects of local gene transfection in CD154 extracellular domain on the survival of renal allografts

目的 观察CD15 4胞外区基因局部修饰大鼠供肾对延长移植肾存活的效能。方法 以CD15 4基因重组腺病毒为载体 ,将CD15 4胞外区基因转入BN大鼠供肾 ,以Lewis大鼠为受体 ,行同种肾移植为转染组 ,并以未转染BN供肾移植给Lewis受体为对照组 ;观察移植肾存活时间和术后肾功能变化。结果 转染组移植肾存活 ( 2 8± 7.3 )d ,较对照组移植肾存活时间 ( 8.6± 1.2 )d明显延长 ;移植组术后血清肌酐较同期对照组明显为低。结论 CD15

Objective To observe the efficacy of local gene transfection in CD154 extracellular domain on the survival of renal allografts. Methods The kidneys of Brown Norway (BN) rats were transfected with CD154 extracellular domain gene recombined adenovirus. The transfected kidneys were transplanted to Lewis rats (transfection group). BN→Lewis kidney transplantation with non transplanted kidneys served as the controls. The allograft survival time and the allograft function between the two groups were compared. Results The allograft survival time of the transfection group was longer than that of the controls significantly [(28±7.3)d vs (8.6±1.2) d, P <0.05]. The serum creatinine of the transfection group at 8 d after operation was lower than that of the controls at 8 d after operation [(67.4±8.1) μmol/L vs (514.9±67.4) μmol/L, P <0.001]. Conclusion Local transfection of CD154 extracellular domain gene can prolong the survival time of renal allografts significantly.