大剂量阿托品对氧化乐果中毒大鼠膈肌功能的影响

The effects of high dose atropine on function of isolated diaphragmatic preparation of rats with omethoate poisoning

目的 探讨大剂量阿托品对膈肌功能的影响。方法 使用MS 30 2生理药理分析仪测定 10只大鼠在两种不同条件下 (对照组用生理盐水皮下注射 ,中毒组用氧化乐果皮下注射 ) ,大鼠反复加入阿托品后对膈肌功能的影响情况。结果 两组大鼠膈肌功能随阿托品累积剂量增至 0 6mg时 ,与阿托品 0 2mg时的膈肌收缩功能相比均明显减弱 (P <0 0 1) ;并随着阿托品累积量增至 0 8mg时 ,膈肌功能接近完全抑制 ;当膈肌中阿托品清除约 5 0min后 ,两组膈肌收缩功能几乎完全恢复正常。结论 反复给予大剂量阿托品可以使大鼠膈肌功能减退 。

Objective The study was designed to examine the influence of high dose atropine on respiratory muscle of rat isolated phrenic diaphragm. Methods Rats were divided into 2 groups: (1)a toxic group administered with 2LD 50 omethoate, SC. (2) a contrast group with 0.9%NS, SC. The function of isolated rat phrenic diaphragm was observed by MS 302 analysis instrument physiologically and pharmacologically. Results In both groups the function of isolated rat phrenic diaphragm was significantly reduced with atropine dose increasing gradually to 0 6 mg ( P < 0.01 ). When the atropine dose amounted to 0 8 mg The function of isolated rat phrenic diaphragm almost disappeared in the picture. When the atropine in the diaphragm was weed out, the function of isolated rat phrenic diaphragm recovered completely in 50 minutes. Conclusion It is suggested that the cause of respiratory muscle paralysis may be related to high dose of atropine.