不同产地阳春砂挥发油气相色谱指纹图谱研究

GC fingerprinting of volatile oils of Amomum villousm from different producing areas

目的 对阳春砂及不同产地引种栽培的阳春砂进行指纹图谱的比较研究。方法 利用GC色谱指纹图谱的方法 ,以道地产区广东省阳春县主产的 10批阳春砂药材为基准 ,对比不同产地引种的阳春砂药材的色谱指纹图谱。色谱条件 :HP 5非极性柱 ,进样口温度 2 30℃ ,检测器 (FID)温度 30 0℃ ,程序升温 70～ 14 0℃ (停 5 0min) ,流速为 0 6 0mL·min- 1 ,分流比为 5 0∶1。结果 从 10批道地产区阳春砂药材挥发油GC指纹图谱中提取 9个共有峰 ,并对不同产地药材进行相似度的比较。结论 阳春砂挥发油气相色谱指纹图谱中各成分均得到了较好的分离 ,可作为阳春砂药材质量评价的专属性的指纹图谱 ,并以此评价不同产地阳春砂的品质。

OBJECTIVE: To apply GC fingerprints to the comparison of Amomum villousm Lour. collected from digerent areas. METHODS: The samples of different areas were compared by application of GC fingerprinting. GC methods were optimized in order to get high quality GC profile. The GC system consisted of HP5890 and FID. The analysis was performed on a HP-5 column (Crosslinked Mehyl siloxame, 30 m x 0.32 mm, 0.25 μn). The temperatures of sample vent and FID were 230°C and 300°C respectively. The column temperature was programmedly elevated from 70°C (5 min) to 140°C (50 min) at 10°C&middotmin-1. The carrier gas was N2. The flow rate was 0.60 mL&middotmin -1. The refractive ratio of distillation was 50:1. RESULTS: The GC fingerprinting was established. The similarity degree of the crude drugs from different producing areas has been carried on. CONCLUSION: A good fingerprint of Amomum villousm has been described. The method is simple and accurate with good reproducibility. The GC fingerprinting with specificity can be applied to the quality control of A. villousm.