人白细胞抗原-DRB基因多态性与特应性皮炎易感性的相关性研究

Study of correlation between polymorphism of HLA-DRB allele and susceptivity of atopic dermatitis

目的 寻找特应性皮炎 (AD)患者易感的人白细胞抗原 (HLA)基因型 ,分析该易感基因的单链构象多态性与正常人的差别。方法 用序列特异性引物聚合酶链反应 (PCR SSP)检测 12 2例AD患者和 80名健康献血员的HLA DRB等位基因型 ,并分析该易感基因的单链构象多态性。结果 外源性AD组和AD合并哮喘及变应性鼻炎组HLA DR3 与正常对照组比较 ,基因频率升高 (RR =5 2 7和3 5 9,均P <0 0 5 ) ,而HLA DR6基因频率降低 (RR =0 10和 0 ,均P <0 0 5 ) ,而HLA DR3 阳性的 16例AD患者中 ,仅 1例的单链构象多态性与正常人不同。结论HLA DR6是AD的抗性基因 ,携带有HLA DR6的个体较不易患AD ,而HLA DR3 是易感基因 ,携带有HLA DR3 的个体 ,较易患AD ,而携带HLA DR3

Objective To look for susceptible and resistant HLA gene of Atopic Dermatitis(AD), analyze single strand conformation polymorphism of the susceptible gene and observe whether the base sequence was different from healthy Method Polymerase chain reaction sequence specific primer(PCR SSP)was used to classify the HLA DRB allele of 122 patients and 80 controls PCR single strand conformation polymorphism(SSCP) was used to analyse the susceptible gene Results The DR 3 frequency of extrinic type of “pure” AD(EAD)group and complicated group was higher than that of the control group ( RR =5 27 and 3 59 respectively P <0 05) DR 6 frequency was lower than that of the control ( RR = 0 10and 0 respectively, P <0 05) There was no significance between instrinic type of “pure” AD(IAD) and control In 16 patients who were HLA DR 3 positive, there was only one patient had SSCP band type different from controls ( P >0 05). Conclusion HLA DR 3 was the susceptible gene of AD, while HLA DR 6 was the resistant gene of AD It indicated that people with HLA DR 3 were reliable to AD, while people with HLA DR 6 were not But there was no base difference between these people and the healthy in HLA DR 3