诱发大鼠自然感染卡氏肺孢子虫肺炎实验研究

Experimental Study on Inducing Pneumocystic Pneumonia in Rats

用地塞米松0.3mg～0.6mg肌注大鼠每周两次,连续9周,第10周改为醋酸泼尼松5mg/次,每周两次,肌注7次。激素总量为42.2mg。诱发大鼠重度肺孢子虫性肺炎,第14周死亡。肺印片查见成熟、未成熟及溃破包囊,切片发现典型的病理改变及肺孢子虫体,作为病原与病理诊断的依据。电镜观察包囊有两种形态,一种为园形包囊,另一种为香蕉形包囊。证明新疆地区具有传播与感染该病原的机会与条件,一但免疫功能低下,可诱发人体卡氏肺孢子虫肺炎而死亡。动物实验成功,为本病提供了进一步研究的条件。

Rats were injected intramuscularly with 0.3mg-0.6mg dexamethasone twice a week for 9 weeks. From the 10th week on ,5mg prednisone acetate was injected intramuscularly twice a week for 7 times. The amount of the two kinds of hormone was 42.2mg for inducing heavy pneumocystic pneumonia in them. The rats were dead on the fourteenth week. The mature, unmature and diabrotic cysts were detected on the slides and typical pathologic changes were identified, is used as the basis for pathologic and etilolgic diagnosis. Electronmicroscope revealed two forms of cytes,i.e. one round in shape and another in bahana-shape. The successful animal experiments is helpful in promoting the study of this disease.