乌桕叶提取物镇痛抗炎作用的实验研究

Analgesic and anti-inflammatory effect of extract of Sapium sebiferum leaves on animal model

目的 :观察乌桕叶提取物 (E SSL)的镇痛抗炎作用。方法 :镇痛实验采用小鼠热板法和扭体法 ,抗炎实验采用二甲苯致鼠耳肿胀法、角叉菜胶致大鼠足趾肿胀法和腹腔毛细血管通透性法。结果 :乌桕叶提取物 0 .5、1.0、2 .0 g·kg-1ig对醋酸引起的小鼠扭体反应有明显的抑制作用 ,抑制率为 5 8.3%～ 34.1% ;1.0 g·kg-1药后 1.5h和 2 .0 g·kg-1药后 1h明显提高小鼠热板痛阈 (P <0 .0 1,P <0 .0 5 )。乌桕叶提取物对多种致炎剂引起的渗出、水肿均有明显的抑制作用 ,1.0、2 .0 g·kg-1ig对二甲苯引起的小鼠耳肿胀、角叉菜胶引起的大鼠足趾肿胀及醋酸引起的小鼠腹腔毛细血管通透性有良好的预防作用 (P <0 .0 1,P <0 .0 5 )。结论 :乌桕叶提取物对实验动物模型具有显著的镇痛抗炎作用 ,且呈明显的量效关系。

AIM: To study the analgesic and anti-inflammatory effect of extract of Sapium sebiferum leave(E-SSL). METHODS: Hot-plate procedure and writhing test in mice were adopted in experimental analgesic model, and anti-inflammatory model of mouse ear edema induced by xylene, rat paw edema induced by carrageenin and exudation of abdominal blood capillaries of mice were applied. RESULTS: The analgesic experiment showed that ig E-SSL at the dose of 0.5,1.0,2.0g·kg -1 had significant inhibitation to writhing response (P<0.01,P<0.05) induced by acetic-acid, the inhibitory rate was 58.3% to 34.1% and the pain threshold in mice with the hot-plate procedure increased obviously after being taken E-SSL for 1.5h in the 1.0g·kg -1 group and 1h in the 2.0g·kg -1 group (P<0.01,P<0.05). In anti-inflammatory experiment, E-SSL had marked inhibitory effect on exudation or edema induced by all three types of inflammation in animal models. ig at the dose of 1.0, 2.0g·kg -1 had good preventive action to mouse ear edema induced by xylene, rat paw edema induced by carrageenin, and exudation of abdominal blood capillaries of mice caused by acetic acid (P<0.01,P<0.05). CONCLUSION: E-SSL has significant analgesic and anti-inflammatory effect on animal models and shows obviously dose-effect ralationship.