摘要
模拟微重力培养是一种全新的组织培养技术,其核心技术是建立动物细胞的三维培养体系,在近10年取得了快速发展。微重力反应器源自美国航空航天局,是一种水平旋转的、无泡的旋转培养仪,可以提供模拟微重力环境,具有充分的氧和营养物质的交换、三维立体结构、低剪切力和独特的流体力学特征等优点。这种悬浮培养技术为多种细胞和组织块的生长和代谢提供良好的培养环境,可以进行高密度的组织培养,并保持所培养细胞的组织分化特异性。
Simulated microgravity culture is a totally new tissue culture technology and develops greatly through the recent ten years. Developed at NASA, microgravity bioreactor is a horizontally rotated, bubble free cell culture vessel with diffusion gas exchange. The system provides a complex three-dimensional, low-turbulence in vitro culture system with abundant oxygenation. The unique environment of low shear forces, high mass transfer, fluid dynamic operating principles and microgravity, provides very good cultivating conditions for growth of many cell types. This suspension culture technique offers the best potential for high-density cell and tissue growth, and tissue-specific differentiation.
出处
《国外医学（生物医学工程分册）》
2004年第3期161-164,共4页
Foreign Medical Sciences(Biomedical Engineering Fascicle)
