摘要
目的 从青岛海星Asteriasrollestoni分离并确定结构新颖的活性化合物 ,并阐明海星中的次生代谢产物的多样性。方法 应用正相和反相色谱包括半制备HPLC方法分离和纯化化合物 ;应用IR ,UV ,MS ,1D和 2DNMR ,以及理化性质确定化合物的结构。结果 从青岛海星Asteriasrollestoni中获得 17种化学成分 ,分别为N7 2′ deoxypseudoxanthosine (1) ,2′ O methyl in osine (2 ) ,2′ deoxyinosine (3) ,2′ O methylguanosine (4 ) ,inosine (5 ) ;thymine (6 ) ,uracil (7) ,thymidine (8) ,deoxyuridine (9) ,2′ O methyluridine (10 ) ,(- ) (1S ,3S) 1 methyl 1,2 ,3,4 tetrahydro β carboline 3 carboxylicacid (11) ,(- ) (1R ,3S) 1 methyl 1,2 ,3,4 tetrahydro β carboline 3 carboxylicacid (12 ) ,(- ) (3S) 1,2 ,3,4 tetrahydro β carboline 3 carboxylicacid (13) ,L tryptophan(14 ) ,L phenylalanine (15 ) ,3 carboxyindole (16 )和p hydroxybenzoicacid (17)。结论 其中 1为新化合物。它们的化学结构经1D ,2DNMR ,以及其它波谱分析 ,结合与已知化合物的波谱和理化数据对照得以确定。化合物 8,9,10为首次从天然资源中获得 ,化合物 14和
Aim To investigate novel bioactive and structural metabolites from marineorganisms. Methods Column chromatography in association with semi-preparative HPLC were used for theisolation of compounds. 1D and 2D NMR, IR, UV, and MS were employed for structure elucidation.Results From the butanol fraction of the 95% EtOH extract of the starfish Asterias rollestoni, a newcompound N^7 -2'-deoxypseudoxanthosine (1), along with sixteen known compounds, 2'-0-methyl-inosine(2), 2'-deoxyinosine (3), 2'-0-methylguanosine (4), inosine (5); thymine (6), uracil (7), thymidine(8), deoxyuridine (9), 2'-0-methyluridine (10), ( ― )-(1S, 3S)-1-methyl-1, 2, 3,4-terrahydro-β-carboline-3-carboxyl-ic acid (11), ( ― )-(1R, 3S)-1-methyl-1, 2, 3,4-tetrahydro-β-carboline-3-carboxylic acid (12) , ( ― )-(3S)- 1, 2, 3,4-tetrahydro-β-carboline-3-carboxylic acid (13), L-tryptophan (14), L-phenylalanine (15), 3-carboxyindole (16), and p-hydroxybenzoic acid (17) , have been isolated. Conclusion Compound 1 is a newnatural product, and compounds 8, 9 and 10 are isolated from natural sources for the first time, andthe known compounds except 14 and 15 are first reported from starfish Asterias rollestoni.
基金
HighTechResearchandDevelopment ( 863 )Pro grarmmeofMinistryofScienceandTechnologyofChi na( 2 0 0 3AA62 0 40 3 ,2 0 0 2AA2 170 81) ,NationalNatu ralScienceFoundationofChina (No .40 1760 3 8,3 0 17110 6)
