EDAG-1在人髓系白血病细胞株中的表达

Study of expression of EDAG-1 in human myeloblast leukemia cells

目的 研究EDAG 1在人髓系白血病细胞株中的表达情况。方法 选取 4种人髓系白血病细胞株分别通过NorthernBlot、PCR、WesternBlot、SouthernBlot了解EDAG 1基因在mRNA、蛋白以及其基因组结构表达情况 ;通过凝胶阻滞实验分析选用白血病细胞系NF κBDNA结合活性。结果 发现向红系分化的K 5 6 2、HEL在mRNA、蛋白水平均高表达EDAG 1基因 ,但未发现突变、扩增和重排。HL 6 0细胞缺失EDAG 1基因。 4种白血病细胞株NF κBDNA结合活性是增强的 ,以高表达EDAG 1基因的细胞株明显。结论 红系白血病细胞株高表达EDAG 1基因 ,该基因的激活可能与编码区结构无关。

Objective To investigate the expression of EDAG-1 in human myeloblast leukemia cells. Methods We selected 4 kinds of huamn myeloblast leukemia cells to explore the expression of mRNA, protein, and the architecture of genome of EDAG-1 with Northern Blot and PCR, Western Blot, Southern Blot respectively; we analyzed the NF-κB binding activity of these cells with EMSA. Results The results showed that mRNA and protein of EDAG-1 gene were highly expressed in erythroleukemia lines (K-562, HEL), while no gene amplification or gene rearrangement and mutation was found in these cells. EDAG-1 gene was deleted in HL-60 cell. Also the NFκB DNA binding activity was enhanced in 4 kinds of leukemia cells, especially in the cells with high expression of EDAG-1. Conclusion mRNA and protein of EDAG-1 gene are highly expressed in erythroleukemia lines;the coding region may not play any role in the mechanisms of EDAG-1 activation.