组织工程化血管的种子细胞在体外培养扩增方法的实验性研究

In vivo experience culture of seed cell for vascular tissue engineering

目的 为培养组织工程化血管的实验研究提供细胞来源。方法 采用酶消化法和组织块法培养人脐静脉血管内皮细胞、人脐动脉血管平滑肌细胞及成纤维细胞 ,并进行细胞的传代、纯化、鉴定以及形态学观察。结果 培养细胞符合血管内皮细胞、血管平滑肌细胞及成纤维细胞的各有形态特征。结论 所培养的细胞有望作为体外构建组织工程化血管的应用。

Objective To provide cells resource for experimental study on vascular tissue engineering. Methods Vascular endothelial cells, smooth muscle cells and human skin fibroblasts were cultured by enzyme digestion and tissue plant methods, and the cells were subcultured, purifiedand, identified and observed the morphology. Results The morphological characteristics of three kinds of cells were accord with those of vascular endothelial cells and smooth muscle cells and human skin fibroblasts. Conclusion The culture cells may be meet the experimental expectations on Vascular tissue engineering as seed cells.