摘要
目的 探索建立急性心肌梗塞动物模型的适宜方法及最佳时间。方法 以 38只Wistar大鼠为研究对象 ,在动物呼吸机的支持下 ,麻醉满意后打开胸腔 ,暴露心脏 ,结扎左冠脉前降支 ,应用心电图动态监测、病理组织切片来确定模型建立情况。结果 心电图动态监测显示ST段在冠脉结扎后呈持续性升高 ;4 0min后可见Q波 ,同时病理组织切片可见结扎区域心肌纤维排列紊乱 ,肌丝断裂溶解 ,细胞核固缩甚至碎裂 ,间质充血水肿 ,有炎性细胞浸润 ;超声检查显示结扎后动物射血分数较结扎前无明显下降 ,无显著统计学意义。结论 通过结扎左冠脉前降支的方法 ,在结扎后 4 0min ,能够建立稳定的心肌梗塞动物模型 。
Objective To explore a feasible method and time for establishment of acute myocardial infarction(AMI) in rats. Method Experiments were performed in 38 Wistar rats with respirator. After anesthetized with phenobarbital sodium, a left lateral thoracotomy was performed through 3-4 intercostals region. The left anterior descending coronary artery (LAD) was ligated by 8-0 sutures to shut the blood supply of myocardium controlled by LAD. Acute myocardial infarction (AMI) created by this technique were confirmed by continuous electrocardiographic monitoring, pathologic section and echocardiographic study. Results After ligation of LAD, ECG displayed ST elevated continuously and pathologic Q wave. Pathologic section suggested myofibers chaotically arranged, myofilament ruptured or lysed, nuclei pyknosis and fragmentation, interstitial hyperemia and edema, and infiltration of inflammatory cells. Meanwhile, echocardiographic study showed ejection fraction didn't descend markedly after ligation of LAD. Conclusion AMI could be created by surgical ligation of LAD,and the animal model would be conducive to the basic study on AMI.
出处
《中国比较医学杂志》
CAS
2004年第3期166-169,共4页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金资助项目 ( 3 0 2 713 81)
重庆市科委资助项目预科发计字 [2 0 0 1] 5 4-4 0
