硫喷妥钠、氯胺酮与安定在实验兔麻醉中的应用

Application of Thiopentone, Ketamine and Diazepam to Combined Anesthesia in Rabbits

目的 探讨硫喷妥钠、氯胺酮与安定复合麻醉在兔实验外科手术中的应用效果。方法 对 1 1 6只外科手术实验用新西兰大白兔经耳缘静脉缓慢推注硫喷妥钠 (2 5 % ,0 6ml kg)行诱导麻醉 ,实验手术前按 1ml kg腹腔注射氯胺酮和安定混合液 1ml kg(氯胺酮 1 2 5 % ,安定 0 1 2 5 % )。术中发现动物有苏醒躁动征象 ,可通过腹腔注射追加氯安合剂 (1ml kg)及硫喷妥钠溶液 (0 5ml kg)。结果 动物无一例死亡。三种药物合用每次可产生麻醉时间为 30～ 4 5min ,可根据手术需要决定追加药物次数。该麻醉能满足手术要求。结论 采用静脉诱导 +腹腔注射可安全快速地获得麻醉效果 ,适用于连续多个动物手术。硫喷妥钠、氯胺酮联合使用 ,麻醉时效同步 ,便于控制麻醉时间。以上方案产生麻醉效果确实 ,过程安全 ,可满足常见兔实验 ,如四肢。

Objective To explore the effect of thiopentone, ketamine and diazepam on combined anesthesia in rabbits.Method Thiopentone(2 5%,0 6 ml/kg) was slowly injected into 116 experimental New Zealand rabbits to perform anesthesia induction,followed by intraperitoneal injection with mixture(1ml / kg) of ketamine (1 25%) and diazepam(0 125%) preoperatively.If palinesthesia and restlessness appeared, intraperitoneal injection with drugs(mixture, 1ml / kg; thiopentone,0.5 ml / kg) would be applied repeatedly.Results No rabbits died.The combined anesthesia sustained 30-45 minutes each time,and satisfied experimental operation.Conclusion Anesthesia effect can be safely and rapidly achieved by anesthesia induction combined with intraperitoneal injection.Thiopentone and ketamine show the similar anesthesia time, which can be easily controlled. The combined anesthesia is suitable for experimental operations on rabbits, such as on limbs,spine or brain.