装饰材料挥发物吸入染毒对大鼠IL-4、IL-5含量及ICAM-1 mRNA表达的影响

Effects of Volatile Organic Compounds Inhalation on the Content of IL-4,IL-5 and the Expression Level of ICAM-1 mRNA in Rats

[目的 ]探讨挥发性有机化合物的吸入和呼吸道变态反应之间的关系 ,为评价装修后室内空气污染提供毒理学依据。 [方法 ]用装饰材料挥发物对雄性SD大鼠静式吸入染毒 3 0d ,测定支气管肺泡灌洗液 (BALF)中白细胞介素(IL 4、IL 5 )含量和肺组织细胞间黏附分子 1(ICAM 1)mRNA表达。 [结果 ]在中浓度染毒组 (甲醛浓度为 0 .81mg/m3 、总挥发性有机化合物 (TVOC)浓度为 84.88mg/m3 ) ,BALF中IL 4含量平均为 12 .95pg/ml,与对照组相比显著升高 ;在高浓度染毒组 (甲醛浓度为 1.5 0mg/m3 、TVOC浓度为 2 99.0 3mg/m3 ) ,BALF中IL 4含量平均为 13 .2 3pg/ml ,IL 5含量平均为2 3 .2 1pg/ml,均显著高于对照组。中高浓度染毒组大鼠肺组织ICAM 1mRAN表达显著增强 ,病理学检查发现肺组织中嗜酸性粒细胞浸润尤为明显。 [结论 ]室内装修可能与呼吸道变态反应性疾病之间有一定联系。

Objective] To study the relationship between volatile organic compounds inhalation and respiratory allergic response,and provide toxicological method for the assessment of indoor air pollution after decoration. [Methods] SD rats were put in exposure chamber for two hours per day for 30 days seccessively where the VOCs volatiled from a block of hardboard painted with four kinds of decoration materials.After one month exposure,the rats were conducted bronchoalverolar lavage,then the content of IL-4 and IL-5 in BALF and the expression level of ICAM-1 mRNA in lung tissue were determined. Pathological slices of lung tissue were also examined. [Results] The mean content of IL-4 in BALF was 12.95 pg/ml at middle concentration group(formaldehyde:0.81 mg/m^3,TVOC:84.88 mg/m^3); while exposed to high concentration of VOCs(formaldehyde:1.50 mg/m^3,TVOC:299.03 mg/m^3),the mean content of IL-4 and IL-5 were 13.23 pg/ml and 23.21 pg/ml respectively,they were all significantly higher when compared with the control. The expression level of ICAM-1 mRNA in lung tissue enhanced significantly and there were also many eosinophil cells infiltrated into lung tissue when exposed to middle and high concentration of VOCs. [Conclusion] There is somewhat relationship between indoor decoration and respiratory allergic response.