两性霉素B/聚乙二醇-聚谷氨酸苄酯纳米球溶血毒性的研究

Haemolytic Evaluation of Amphotericin B Loaded PEG-PBLG Nanospheres

目的 :考察两性霉素B/聚乙二醇 聚谷氨酸 (AmB/PEG PBLG)纳米球是否能降低AmB的溶血毒性。方法 :超微透析法制备AmB/PEG PBLG纳米药球 ,用紫外分光光度法测定样品的溶血率。结果 :AmB/PEG PBLG纳米药球平均粒径 14 2 1nm ,载药量 2 7 5 5 % ;溶血率从低到高依次为 :PEG PBLG空白纳米球、脱氧胆酸钠、AmB/PEG PBLG纳米药球、AmB注射液。结论 :AmB/PEG PBLG纳米药球能有效降低AmB的溶血毒性。

AIM:To investigate whether the amphotericin B (AmB) loaded PEG-PBLG nanospheres decreased the hemolytic toxicity.METHOD:AmB loaded PEG-PBLG nanospheres were prepared by diafiltration method.The haemolytic percentage was measured by UV.RESULT:The mean particle size was 142.1 nm and loading drug content was 27.55%.The haemolytic percentage from low to high were PEG-PBLG nanospheres,sodium deoxycholate,AmB loaded PEG-PBLG nanospheres,AmB injection.CONCLUSION:PEG-PBLG nanospheres are promising carriers to decrease the haemolytic toxicity of AmB.