病理浓度哇巴因作用于血管内皮细胞后的基因表达谱改变

Gene expression analysis of human vascular endothelial cells treated by ouabain in pathological concentration

目的 用基因芯片研究病理浓度哇巴因作用于血管内皮细胞后的基因表达谱改变。方法 以包含 84 6 4条人类基因的DNA芯片检测血管内皮细胞受 1 8nmol/L哇巴因活化后的基因表达谱。结果 血管内皮细胞受哇巴因作用 2h后 ,12 9条基因出现表达差异 ,其中上调的共有 2 6条。结论 通过基因表达谱研究提示病理条件下哇巴因对血管内皮细胞的作用涉及DNA转录调控、蛋白质翻译合成。

Objectives To study the gene expression of human vascular endothelial cells (HUVEC) treated by ouabain in pathologicall concentration. Methods The response of endothelial cells to ouabain of 1.8 nmol/L was explored with a complementary DNA microarray representing 8 464 different human genes. Results The results of mRNA profiles analysis indicated that 129 of genes were differently expressed, 26 were upregulated. Conclusions The pathological role of ouabain on HUVEC may be involved in the controlling of DNA transcription, protein translation, metabolism and signal transduction.