人工肝支持系统治疗前后HBV DNA水平的变化

Changes of HBV DNA before and after the treatment of artificial liver support system

目的探讨重型乙型病毒性肝炎HBVDNA的复制情况及其在人工肝支持系统治疗中的变化。方法采用荧光定量PCR方法检测重型乙型病毒性肝炎患者人工肝前后HBVDNA水平。结果人工肝治疗前后病人血清HBVDNA定量(log值)分别为(6.20±0.53),(5.33±0.47)(=36,<0.01);治疗后血清总胆红素、直接胆红素、总胆汁酸、丙氨酸转氨酶等明显下降(=78,<0.01),凝血酶原时间明显缩短(=78,<0.01)。结论人工肝支持系统治疗可降低重型乙型病毒性肝炎HBVDNA水平并能明显改善其肝功能。

Objective: To investigate the replication capacity of HBV DNA and evaluate the changes of HBV DNA before and after the treatment of artificial liver support system in the patients with severe viral hepatitis. Methods: The copies of HBV DNA were detected by quantitative polymerase chain reaction (FQ-PCR) before and after the treatment of artificial liver support system in the patients with severe viral hepatitis. Results: HBV DNA quantity (log value) had a reduction from (6.20±0.53) to (5.33±0.47) (n=36, P <0.01) after the treatment of artificial liver support system, and the levels of serum total bilirubin, direct bilirubin, total bile acid and aminotransferase were significantly decreased (n=78, P <0.01), and the prothrombin time was significantly shortened (n=78, P <0.01) after therapy. Conclusions: The treatment of artificial liver support system can reduce the level of serum HBV DNA and improve greatly liver function in the patients with severe viral hepatitis.