大豆生理活性肽的研究(Ⅱ)——抗氧化性和ACE抑制活性的初步研究

Soybean Bioactive Peptides(Ⅱ)——Antioxidant activity and ACE inhibitory activity of the hydrolysates

采用AS1.398和Alcalase两种蛋白酶,制备了水解度为10%24%的大豆多肽,对其抗氧化性、ACE抑制活性和相对分子质量的分布进行了研究,结果表明:采用AS1.398酶水解的DH为12%的产品抗氧化活性最高,添加量为6mg/mL时使亚油酸的氧化诱导期延长2.92倍,其相对分子质量分布在1000以上的组分较多;采用Alcalase酶水解的DH为14%的大豆多肽产品,ACE抑制活性最高,IC50为0.144mg/mL,其相对分子质量分布大多在200600之间.

The experiments investigating the properties of antioxidant activity,ACE inhibitory activity, and molecular weight distribution of the soybean polypeptides with DH of 10%~24% producted by AS1.398 and Alcalase, were conducted.The results indicated that the sample producted by hydrolyzing SPI with AS1.398 at DH 12% had the best antioxidant activity among all the other samples. The oxidant induction period of linoleic acid was extended to 2.92 times of that of the blank when the sample concentration was 6 mg/mL.Whereas, the sample producted by hydrolyzing SPI with Alcalase at DH 14% had highest ACE inhibitory activity among all the samples, and its IC_(50) was 0.144mg/ml.From molecular weight distribution measurements,it could be found that the hydrolysates with higher antioxidant activity were those having more peptides(consists of about 10 amino acid units, MW higher than 1 000), and the hydrolysates with higher ACE inhibitory activity were mainly those of molecular weight ranging from 200 to 600.