摘要
目的 采用高效液相色谱法测定鹿茸生新胶囊中芍药苷的含量以控制该制剂的质量。方法 以C18化学键合硅胶柱分离芍药苷;乙腈 - 0 1%磷酸(18∶82 )为流动相;UV检测波长 2 30nm。结果 芍药苷峰与其它分峰的分离度为 8 2;理论塔板数以芍药苷峰计算为 12 35 0;加样回收率为 97 9% ( RSD为 1 2 %,n =5 );芍药苷在 0 2 5～ 2 5 μg范围内进样量与面积值呈良好的线性关系。结论 本法测定鹿茸生新胶囊中芍药苷的含量,结果准确,重复性好。
Objective Determination and quality control of Paeoniflorin in Lurong Shengxin Capsules by RP-HPLC. Methods Paeoniflorin was separated by C 18 column with acetonitrile-1%(18∶82) as mobile phase. Determination wave-length was 230nm. Results Resolution between Paeoniflorin peak and other peaks was 8.2, and number of theoretical plates calculated for Paeoniflorin was 12350. The recovery rate of added sample was 97.9%, and RSD was 1.2%(n=5). There was a good linear relationship between the concentration of Paeoniflorin and absorption area value in the range of 0.25～2.5μg. Conclusion This method was highly reproducible and accurate for the determination of Paeoniflorin in Lurong Shengxin Capsules.
出处
《齐鲁药事》
2004年第4期19-20,共2页
qilu pharmaceutical affairs
