间变性大细胞淋巴瘤中ALK蛋白及EB病毒基因产物的表达

The Expression of ALK Protein and Epstein-barr Virus Gene Products in Anaplastic Large Cell Lymphoma and Its Significance

目的 研究间变性大细胞淋巴瘤 (AL CL)中 AL K蛋白的表达特点及与 EBV的关系。方法 采用免疫组织化学 SP法检测 AL K蛋白表达 ,EBV基因转录产物 EBER1 / 2以 DNA- RNA原位杂交方法检测。结果 AL K蛋白在 2例皮肤原发 AL CL 均为阴性 ,2 0例系统性 AL CL 中有 1 2例 (6 0 % )阳性 ,其中普通型 8例 ,淋巴组织细胞型 3例、小细胞型 1例 ,AL K蛋白的表达在各组织学亚型中无显著性差异。表达模式主要为细胞浆与细胞核均为棕黄色 ,但其中有 2例仅为细胞浆着色 ;AL K蛋白阳性组和阴性组年龄差异具有显著性 (P<0 .0 5 ) ,AL K蛋白阳性组年龄显著低于阴性组。所有 2 2例 AL CL 病例 EBER原位杂交均为阴性。结论 AL K蛋白的表达在AL CL 中有很高的发生率 ,尤其是低年龄的患者。本研究未发现 AL CL 与

Objective To investigate the characteristic of ALK protein expression in anaplastic large cell lymphoma(ALCL) and the relationship between ALCL and Epstein Barr virus(EBV). Methods Histopathological method was used to study the morphological characteristic. Immunohistochemical staining was used to detect ALK protein and other antigen. EBV encoded EBER was detected by in situ hybridization. Results All 2 cases of primary cutaneous ALCL were ALK protein negative. 20 cases of systemic ALCL were found to be ALK protein positive in 12 cases (60%), which included 8 cases of common variant, 3 cases of lymphohistiocytic variant and 1 case of small cell variant; the expression of ALK protein showed no significant difference between these 3 variants ( P >0.05). The ALK protein expression pattern was characterized by the brown stained nucleus and cytoplasm of the cells, but in 2 cases only the cytoplasm was stained. The patients with ALK protein expression were younger than those without ALK protein expression( P <0.05); all 22 cases were EBER negative. Conclusion High incidence of the expression of ALK protein was seen in ALCL, especially in ALCL of younger patients. No relationship was observed between EBV and ALCL in this study.