肌动蛋白在新生猪RTE细胞ATP缺乏损伤机制中的作用

Function of Actin in Renal Tubular Epithelial Cell of Newborn Swine During ATP Deficiency

目的 研究肌动蛋白 (actin)在 ATP缺乏时新生猪肾小管上皮细胞 (RTE)损伤中的作用 ,探讨新生儿窒息时肾功能不全的发生机制。方法 建立四川白猪 I系新生猪 RTE细胞 ATP缺乏细胞模型后 ,以流式细胞仪检测正常以及 ATP缺乏不同时间段 RTE细胞中肌动蛋白含量的变化。结果 随着 ATP缺乏时间延长 ,G-actin和 F- actin含量在 ATP缺乏 1 min均出现变化 ,G- actin先下降后上升 ;F- actin则持续下降。结论 ATP缺乏时 RTE细胞中 G- actin和 F- actin含量的变化导致细胞骨架蛋白破坏 ,使细胞骨架稳定性破坏 ,可能是窒息时肾功能损伤的机制之一。

Objective To investigate the variations of actin of newborn porcine Renal Tubalar Epithelial(RTE) cell during ATP deficiency and shed light on the possible mechanisms of renal deficiency during newborn asphyxia. Methods After the establishment of ATP deficiency cell model, the ATP content of the RTE cell of the control and hypoxic groups was determined. And the variations of actin in newborn porcine RTE cell during ATP deficiency were detected using flow cytometry. Results It was found that the ATP content of new born porcine RTE cell decreased with the continuance of hypoxia, and the changes in G actin and F actin contents of RTE cell both appeared at the time of one minute ATP deficiency. The G actin decreased first and then increased, and the F actin decreased step by step. Conclusion As the ATP deficiency time elongated, G actin of the newborn porcine RTE cell decreased first and then increased, and the F actin decreased step by step. This may destruct the cell bone skeleton of the newborn RTE cell and maybe one of the important mechanisms of renal deficiency during newborn asphyxia.