中药WPY对急性坏死性胰腺炎大鼠胰腺及肺组织表达ICAM-1及MPO活性的影响

Impact of Traditional Chinese Medicine WPY on ICAM-1 Expression and MPO Activity in Pancreas and Lungs of Rats with Acute Necrotizing Pancreatitis

目的 探讨中药 WPY对急性坏死性胰腺炎 (ANP)大鼠胰腺及肺组织中细胞间粘附分子 - 1 (ICAM-1 )表达及细胞过氧化物酶 (MPO)活性的影响。方法 35 g/ L 牛磺胆酸钠逆行胰胆管注射诱发大鼠 ANP及中药WPY治疗后 ,用免疫组化技术观察胰腺、肺组织 ICAM- 1的表达改变 ,测定胰腺、肺组织髓过氧化物酶 (MPO)活性。结果 ANP后 3h胰腺的 ICAM- 1表达及 MPO活性明显增强 ,1 2 h达高峰。ANP后 6 h肺组织的 ICAM- 1表达及 MPO活性明显增强 ,1 2 h达高峰。中药 WPY治疗组胰腺及肺组织 ICAM- 1表达及 MPO活性下降 ,在 1 2 h时相点与模型组比较具有统计学意义。结论 中药 WPY可以减轻胰腺及肺组织 ICAM- 1的表达 ,使

Objective To explore the character of the ICAM 1 induced change of polymorphonuclear neutrophil (PMN) in the pancreas and lung of rats with acute necrotizing pancreatitis (ANP) and to evaluate the effects of Chinese medicine WPY on ANP. Methods ANP model was induced by retrograde injection of 3.5% sodium taurocholic acid into the biliopancreatic duct of Wistar rats. Expression of ICAM 1 at different time points in pancreas and lung was assessed by immunohistochemistry. The quantity of polymorphonuclear neutrophil (PMN) in tissues was measured by the level of myeloperoxidase activity (MPO). In addition, the indices above were also observed after the administration of WPY. Results In the ANP group, ICAM 1 expression and MPO activity were evidently up regulated at 3 h in pancreas and at 6 h in lung, and both reached their highest level at 12 h. In the WPY treated group, ICAM 1 expression and MPO activity in pancreas and lung were significantly decreased at 12 h. Conclusion The numerous PMNs infiltration mediated by ICAM 1 plays an important role in the damage to pancreas and lung caused by ANP. The traditional Chinese medicine WPY can attenuate ICAM 1 expression and MPO activity in pancreas and lung during ANP.