SARS冠状病毒M蛋白基因的克隆、鉴定及原核表达载体的构建

Cloning, identification and construction of the prokaryotic expression vector of the M gene of SARS-CoV

目的 通过逆转录聚合酶链反应 (RT PCR)扩增SARS冠状病毒M蛋白基因 ,并构建M蛋白基因的原核表达载体 ,为进一步研究其功能做准备。方法 提取SARS冠状病毒总RNA ,通过RT PCR对其M蛋白基因进行扩增 ,并构建M蛋白基因的原核表达载体。结果 获得了SARS冠状病毒M蛋白基因的编码序列并将其克隆入原核表达载体pET30a。结论 成功构建SARS冠状病毒M蛋白基因的重组表达质粒 。

Objective To amplify the M gene of SARS CoV and construct the prokaryotic expression vector for further study. Methods Total RNA was extracted from the cell culture supernatant of SARS CoV, and the M gene was amplified by RT PCR. The fragment was inserted into the prokaryotic expression vector. Results The specific M gene fragment was amplified from SARS CoV and inserted into the expression vector pET30a. Conclusion The successfully construction of recombinant expression vector of M gene from SARS CoV will benefit for further research.