192-Saporin对SD大鼠股骨发育的作用研究

Effects of systemic injection of 192-Saporin of development of rat long bone

目的研究胆碱能神经系统对骨发育的影响。方法用192-Saporin抑制SD乳鼠胆碱能神经系统的发育和功能,每2d腹腔注射1次,持续2周,股骨进行大体测量和生物力学检测,坐骨神经行Loyez髓鞘染色。结果药物处理对乳鼠生理状态和运动能力无明显影响,处理前后处理组与对照组体重比较无显著性差异(P>0.05);处理组股骨中段冠状面皮质直径小于对照组(P<0.01),直径/全长比值无显著性差异(P>0.05);处理组股骨最大抗折应力小于对照组(P<0.05),刚性比较则无显著性差异(P>0.05);处理组坐骨神经轴突排列较紊乱,密度较对照组低(P<0.01)。结论处理组股骨发育较慢但发育比例正常,以及矿化程度未受影响,提示胆碱能神经系统可能在成骨细胞的增殖过程中起重要作用。

Objective To study the effects of the cholinergic nerve system on long bone development. Methods 192-Saporin was injected into newborn SD rats through enterocoelia every other day for 2 weeks. The rat weights were recorded before and after treatment. After the injection, the rats were killed and their sciatic nerves and unilateral femurs were isolated. Their femurs were investigated by both gross measurement and biomechanical investigation, and the development of sciatic nerves was observed pathologically. Results The physical behaviors and weights of rats in the treatment group were not much different from those in the control group (P >0.05). The differences of femoral diameter between the treatment and control groups were significant (P< 0.01) but the diameter/length ratio comparison was not significant (P >0.05). The average stress force of femoral shaft in the treatment group was lower than that in the control group (P< 0.05), but the rigidity differences between the 2 groups were not remarkable (P >0.05). The sciatic nerve in the control group was more admirable in structure and had more axons (P< 0.01) than that in the treatment group. Conclusions Long bone development is regulated by cholinergic nerve system to some extent. We can make an assumption that during long bone development, anti-cholinergic-nerve treatment may decrease the proliferation of osteoblasts while the calcification of osteoblasts is intact.