共滴定法制备纳米羟基磷灰石/胶原复合材料及其性能

Preparation and Characterization of Nano-hydroxyaptite/Collagen Composite Synthesized by Simultaneous Titration

以饱和Ca(OH)2溶液的上清液、磷酸和可溶性胶原为原料,在37℃、中性条件下,采用共滴定法制备纳米羟基磷灰石/胶原复合仿生骨修复材料。用XRD、FTIR、扫描电镜、透射电镜对材料晶相组成、微观形貌、结构、化学组成、晶粒大小进行分析。复合材料的抗压强度用电子万能实验机测试。结果表明:复合材料由弱结晶的纳米尺度(5nm×60nm～20nm×80nm)的羟基磷灰石和胶原纤维组成。复合材料在晶相组成、化学组成和晶体尺寸上类似于天然骨。复合材料的抗压强度(168.85MPa)和弹性模量(5.87GPa)介于致密骨和牙本质之间。此复合材料有望成为理想的骨修复材料。

Nano-hydroxyapatite/collagen biomimetic composites for bone repair were synthesized from the supernatant of saturated Ca(OH)2 solution, phosphoric acid, soluble collagen, by simultaneous titration, at 37°, neutral conditions. Crystallite phase, micro-morphology, structure, chemical composition, crystallite size of the composite were characterized by XRD, FTIR, SEM and TEM. The compressive strength of the composite was measured using a electronic universal-testing machine. The results showed that the composite consisted of nanosize hydroxyapatite (5 nm ×60 nm-20 nm × 80 nm) with low crystallinity and collagen fiber. Crystallite phases, chemical composition, crystallite size of the composite were similar to that of natural bone. Compressive strength and modulus of elasticity of the composite were 168.85 MPa and 5.87 GPa, respectively, which were higher than that of cortical bone and lower than that of dentine. It is expected that the nano-hydroxyapatite/collagen biomimetic composites will be ideal bone repair substitutes.