梨枣组织培养的研究

Study on Tissue Culture of Pear Jujube

从梨刺茎段培养直接诱导了不定芽,并形成了完整的试管苗。试验表明,在MS+ZT1.75mg·L-1+KT2.0mg·L-1培养基中,梨枣茎段可以分化不定芽,低含量的NAA和AgNO3可以促进不定芽的形成。在MS+ZT1.75mg·L-1+KT2.0mg·L-1+NAA0.05mg·L-1+AgNO32.0mg·L-1培养基中,芽的增殖系数最高,达到2.25倍。在1/2MS+IBA0.08mg·L-1培养基上,试管苗的生根率达到95.0%。

Adventitious shoot was induced from the stem fragment of pear, and forms a complete vitro-seedling. The test indicated that the stem fragment of pear jujube can differentiate adventitious shoot in the medium composed of MS, ZT(1.75 mg·L^(-1)) and KT(2.0 mg·L^(-1)), and lower content of NAA and AgNO3 can promote the formation of adventitious shoot. In the medium composed of MS, ZT(1.75 mg·L^(-1)), KT(2.0 mg·L^(-1)), NAA(0.05 (mg·L^(-1))) and AgNO3(2.0 mg·L^(-1)), the propagating coefficient of shoot is the highest which is 2.25 times as much as usual. In the medium composed of half MS and IBA(0.08 mg·L^(-1)), the growing rate of root can reach 95.0%.