摘要
以湘林1号和湘林4号油茶优良无性系近成熟种子为材料,总结了构建油茶种子cDNA文库过程中存在的一些关键问题和应采取的对策,为今后油茶和其它油料植物种子的mRNA提取、构建高质量的cDNA文库及ESTs文库等提供技术参考和理论指导。
The key questions and solutions of cDNA Library construction from the approximately ripe seed of Camellia oleifera collected from Xianglin 1st and 4th, which were excellent asexual forest, were summarized. The technological reference and theoretical direction for isolating mRNA, and construction of cDNA and ESTs of Camellia oleifera and other oil plants were offered.
出处
《经济林研究》
2004年第2期53-55,共3页
Non-wood Forest Research
基金
国家自然科学基金资助课题"油茶EST文库构建及重要基因的分离与鉴定"
教育部骨干教师资助课题"油茶cDNA文库构建及探针筛选"项目的部分内容。
关键词
油茶种子
CDNA
文库构建
质量
nonwood forest
Camellia oleifera
cDNA
mRNA
library construction
