摘要
用兔病毒性出血症抗原免疫产蛋鸡,收集高免蛋,精制蛋黄抗体(IgY),采用血凝抑制试验(HI)测定IgY的最高滴度为151og2。通过IgY的被动免疫保护试验证实,IgY滴度稀释至131og2时肌肉注射体重2.0～2.5kg的健康非免家兔5.0mL/只,可抵抗兔病毒性出血症强毒RC株1.0mL(血凝价HA为91og2)的攻击。临床应用效果显示:IgY(HI为13log2)对发病兔进行紧急防治,治愈率为84.38%～92.86%,能迅速控制疫情。
A flow full- grown hens were immunized with inactivated rabbit haemorrhagic disease virus(RHDV).After a boosting process eggs were collected and used to extract the special yolk antibody(IgY).The maximum titer of this preparation reached 15 1og2 by hemagglutination inhibition assay(HI).It was confirmed through passive immunization of IgY that healthy nonimmunized rabbits whose weight varied during 2.0kg and 2.5kg were intramuscularinjected with IgY 5.0mL(HI=13 1og2),they could resist the attact of 1.0 mL(HA= 9 1og2)RHDV- RC.The clinical application effect of IgY(HI=13 1og2)showed the cure rate was 84.38 to 92.86 percent to diseased rabbits and they could control the epidemic situation immediately.
出处
《中国养兔》
2004年第4期20-22,共3页
Chinese Journal of Rabbit Farming
