rhTNF-α,rhIL-1-α,PHA-LCM和顺式维甲酸对U_(937)细胞诱导分化的体外研究

In Vitro Study of Differentiation of U_(937) Cells Induced by rhTNF-α, rhIL-1-α, PHA-LCM and c-RA

观察了3种细胞因子和顺式维甲酸(c-RA)在体外对U_(937)细胞增殖与分化的影响。结果表明:rhTNF-α、PHA-LCM和c-RA均能抑制U_(937)细胞的增殖并诱导其向成熟细胞分化,作用方式呈剂量和时间依赖性,而且rhTNF-α和c-RA合用后作用增强。rhIL-1-α单独对U_(937)细胞的增殖与分化没有影响,但可增强c-RA的诱导分化作用。[~3H]-TdR掺入实验表明,rhTNF-α、PHA-LCM和c-RA通过抑制U_(937)细胞合成DNA而使其增殖停滞。结果揭示:合理联合应用分化诱导剂能互相增强作用,对进一步提高白血病细胞的诱导分化效应以及临床治疗有一定的参考价值。

In this study, several physiological factors were studied for their effects on the growth and differentiation of U937 cells in vitro .The results showed that (1)rhTNF-α,PHA-LCM and c-RA exerted a dose and time-dependent suppression of proliferation of U937 cells; (2) the synergistic effect oc-cured when rhTNF-α was combined with c-RA in the cell culture; and (3)rhIL-1-α augmented the differentiation of U937 cells induced by c-RA though it had no effect on the cell line by itself. According to the [3H]-TdR incorporation, it can be concluded that the reduction of DNA synthesis of U917 cells by rhTNF-α ,PHA-LCM,and c-RA is attributed to the inhibition of the growth. The results might have clinical significance in the induction of differentiation of leukemia cells.