抗瘤抗体对LAK细胞抗肿瘤活性的增强作用

Enhancing Effect of Anti-tumor Antibody on the Cytotoxicities of Lymphokine- activated KiUer Cells

应用体外培养的肿瘤细胞株K562和P815每只以5×10~6个/d剂量分别腹腔免疫昆明种小鼠,获得2种肿瘤细胞抗血清。经ELISA检测,证明2种抗血清中抗瘤抗体效价均在1250以上。用不同浓度IL-2诱导的LAK细胞和相应的肿瘤靶细胞结合早期加入该靶细胞抗血清,[^(125)I]UdR释放分析测杀伤活性,发现K562抗血清不但可以显著增强癌症病人外周血淋巴细胞抗K562活性,而且P815、K562抗血清分别能显著增强鼠源和人源性LAK细胞抗P815和K562活性。这种LAK细胞活性增强作用是由抗血清中抗瘤抗体而不是补体和其他细胞因子所介导。

Adult Kunming mice were immunized with K562 and P815 by a single intraperitoneai injection at a dose of 5×104 cells/murine/d. Anti-P815 and anti-K562 antisera were prepared. Contents of anti-tumor antibodies in the antisera were proved to be high by means of ELISA assay. Splenocytes of C57BL/6 mice and peripheral blood lymphocytes (PBL) from cancer patients were incubated with 125-1000 U/ml IL-2 for 4d. Anti-P815 activity of the LAK cells from C57BL/6 splenocytes was greatly enhanced in the presence of anti-P815 anti-serum at the early stage of culture of LAK and P815 together in 18h [125I]UdR release assay. Anti-K562 activity of LAK cells from PBL was also greatly enlianced by anti-K562 antiserum when the antiserum was added at the early stage of culture. Anti-tumor antibodies in the antisera, not complement and other cytokines, were responsible for the augmentation of NK and LAK cell activities.