急性白血病LAK细胞的诱导与长期培养后其杀伤活性的动态观察

Induction of LAK Cells in Acute Leukemia Patients and Kinetics of LAK Activity in Long Term Culture in Vitro

将急性白血病(AL)患者外周血单个核细胞(PBMNC)在1000 U/ml IL-2的条件下培养,结果显示:正常人LAK细胞一般在IL-2诱导后3 d即具有显著杀伤活性,并以3～5 d之间LAK细胞活性最强;而AL患者的LAK细胞,第5天表现出LAK细胞活性,第15天达到高峰,其高峰较正常人明显延迟。LAK细胞免疫表型分析显示,经IL-2培养后,CD_8^+,CD_(16)^+亚群细胞以第5天开始升高,第3周达到高峰;而CD_4^+亚群则从第5天开始降低,第3周最低。表明LAK细胞杀伤活性与CD_8^+亚群、CD_(16)^+亚群的比例呈正相关,而与CD_4^+亚群比例呈负相关。

Peripheral blood mononuclear cells (PBMNC) isolated from patients with acute leukemia (AL) and from normal controls were cultured in medium containing 1000 units/ml of recombinant interleukin 2 (IL-2). Marked LAK activity was induced on the third culture day in the normal controls,, with the highest cytotoxicity appearing between day 3 and 5 whereas induction of LAK activity in the AL patients began on the 5th day of culture, with the peak level appearing at day 15, showing that the peak of LAK activity was significantly delayed in AL. LAK cells surface phenotyping tests showed that CD8+ and CD16+ positive cells began to increase significantly from day 5 and reached the highest level at week 3, whereas CD4+ subclass began to decrease on day 5 and dropped to the nadir at week 3. The proportion of CD8+ and CD16+ cells were positively cor related with LAK activity, but, that of the CD4+ cell was inversely related with the LAK activity.