摘要
结合电聚合膜和纳米金自组装技术,提出了一种新的生物分子固定化方法,研制成一种检测抗胰蛋白酶的压电免疫传感器。通过在石英晶振金电极表面电聚合邻苯二胺膜,再在膜表面自组装一层纳米金粒,以静电吸附作用固定抗体(抗原),实现对相应抗原(抗体)的检测。利用扫描电镜技术,从形态上考察了晶振金电极上自组装纳米金后的表面形貌。研究了抗体的固定化条件,探讨了传感器的响应与再生性能。结果表明,这种固定化方法对所固定的生物分子的生物活性影响小,传感器的测定灵敏度高,响应性能和再生性能较好。
In this paper,a novel method for biomolecule immobilization is proposed based on the combinaˉtion of electropolymerized films(Eps)and self_assembled nanostructures.The method was applied to the development of an immunosensor for antitrypsin(α 1 -AT)detection in human serum.An electropolymerˉized o_ phenylenediamine film was immobilized on the surface of a quartz _ crystal microbalance(QCM).Then a self_assembled nano_gold layer was applied on the electropolymerized film.Finally the antibodies of the antitrypsin(Anti_α 1 -AT)were electrostatically adsorbed onto the QCMsurface for the detection of antitrypsin via immunoreaction.The surface morphologyof the QCMwas studied with scanningelectron miˉcroscopy(SEM)after nano_gold modification.The antibody immobilization conditions have been investigatˉed in details.As compared to the glutaraldehyde binding procedure,the nano_gold self_assembly proceˉdure provides higher antibody activity and larger frequency response to immunoreactions.It is concluded that this immunosensor has the advantages of higher sensitivity,improved selectivity and better regeneraˉtion performance.
出处
《分析测试学报》
CAS
CSCD
北大核心
2004年第4期1-4,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(20075006
29975006
20205004)
关键词
邻苯二胺电聚合膜
纳米金
固定化方法
压电免疫传感器
Electropolymerized o-phenylenediamine films
Nano-gold
Method of immobilization
Piezoˉelectric immunosensors