巯基棉分离-氢化物原子荧光光谱法测定中药中不同形态的砷

Determination of Arsenic Species in Traditional Chinese Medicines by Hydride Generation-Atomic Fluorescence Spectrometry Using Separation Technique with Sulphhydryl Cotton Fiber

建立了巯基棉分离吸附-氢化物发生-原子荧光光谱法测定中药中不同形态砷的分析方法。在1mol／L盐酸介质中,利用巯基棉实现了As(Ⅲ)和As(Ⅴ)的分离。研究了试剂浓度、不同增感剂等对砷荧光强度的影响和砷(Ⅴ)的还原条件,利用本法成功地对5种中成药中的砷进行了形态分析。方法检出限为64.3ng／L,RSD为1.12%,回收率为90%～116%。

A method for the determination of arsenic species by hydride generation-atomic fluorescence spectrometry using separation technique with sulphhydryl cotton fiber(SCF)was developed.In a medium of1mol/L HCl,As(Ⅲ)could be separated from As(Ⅴ).The concentration of reagents and different sensitizers thatinfluenced the atomic fluorescence signal intensityand the reduction of As(Ⅴ)were investiˉgated.A detection limit of64.3ng/L,a relative standard deviations of1.12%and a recovery range of90%～116%were obtained.The proposed method was applied to the determination of arsenic species in five kinds of Traditional Chinese Medicines.