微柱高效液相色谱法分离八角茴香挥发油成分

Separation of Volatile Constituents in Illicillm Verum Fruit by Micro-column Liquid Chromatography

利用微柱高效液相色谱法(μ-HPLC)对八角茴香果实挥发油成分进行了分离,对固定相类型、流动相组成及流速等条件进行了优化。结果表明,选用氰基分析柱(250mm×0.32mmi.d.,5μm),正己烷-乙腈-二氯甲烷(80∶8∶12,体积比)为流动相,流速为2μL·min-1,八角茴香果实挥发油成分在微柱液相色谱上分离效果最好。在优化的实验条件下,对实际样品中反式-茴香醚、茴香醛等成分进行了定量。本实验为以后的μ-HPLC与毛细管气相色谱联用分析植物挥发油奠定了基础。

Constituents in the essential oil of illicillm verum fruit were separated by HPLC with a packed capillary column.Factors affecting the separation performance,such as the composition and flow rate of the mobile phase,as well as the stationary phase,were investigated.The results showed that good separaˉtion can be achieved by using a capillary column packed with5μm cyano_bonded silica and a mobile phase consisting of n_hexane,acetonitrile and dichloromethane in a volume ratio of80∶8∶12at a flow rate of2μL·min -1 .Quantitation of main components,i.e.,trans_anethole and anisoldehyde,was perˉformed by the'external standard'method.