猪伪狂犬病血清抗体两种检测方法的比较

Comparison of two methods for detecting antibodies against porcine pseudorabies

应用酶联免疫吸附试验和免疫金标检测试纸两种方法 ,同时对 64头份猪血清样品进行猪伪狂犬病血清抗体检测 ,试验结果显示 :两种方法检测的 64头份猪血清中伪狂犬病抗体阳性率均为 62 5 % (40 / 64) ,符合率达到 1 0 0 %。两种方法都具有微量、特异、准确的优点。前者需要用酶标仪等仪器 ,试验时间相对较长 ,成本高 ,但能用准确的数字表达抗体水平 ;后者不需要任何仪器设备 ,试验时间短 ,成本低 ,适用于基层兽医站、养殖场使用 ,以及大面积开展猪伪狂犬病抗体检测的使用。

pig sera were detected by ELISA kit and colloidal gold strip. They had the same results and 40 positive in 64 sera (62.5%). They appear to be a little、specific and exact methods for detecting porcine pseudorabies. ELISA kit need expensive equipment and reagents、longer experiment time, but can show antibody level at a figure. Colloidal gold strip don't need equipment、short-time detection and low expense, it quite may be applied for basic veterinary station and pig farm.