荷叶黄酮的乙醇提取工艺优化研究

Optimum extracting technology of flavonoids in lotus leaves with ethanol

研究了荷叶中主要生理活性物质黄酮的乙醇提取工艺。将传统的正交试验和人工神经网络方法相结合,提出了一种新的试验数据分析、处理和优化方法,可以充分挖掘试验信息,定量分析因素变化规律和寻找参数最优组合。该方法应用于荷叶黄酮的提取工艺优化中,获得了乙醇提取的优化工艺条件,即固液比1∶30、提取温度75℃、提取时间1h和乙醇浓度50%。该优化工艺条件,在实际生产中应用,不仅减少了生产成本,降低了能耗,而且提高了黄酮的浸提率,取得了较好的成效。

The extracting technology of flavonoids in lotus leaves with ethanol was studied. Combined artificial neural network (ANN) with traditional orthogonal design, a new method of test data analyzing, processing and optimizing was put forward. With this method the test information can be fully used, the change regularity of each factor can be analysed and the optimum combination can be found. By using this new method, the optimum extraction condition was obtained as follows: extracting temperature 75℃, ethanol concentration 50%, ratio of solid-liquid 1∶30 and extracting time 1 h. Applying this extracting technology, the costs of production was reduced, the energy consuming was decreased, and the flavonoid content was increased. The results show that this new method is reasonable and practicable.