壳聚糖酶生产菌的产酶工艺条件研究

Primary study on technological conditions for chitosanase-producing microorganism

壳聚糖是自然界中唯一一种带阳离子的能生物降解的高分子材料,已广泛应用于农业、医药、食品等领域。其降解产物甲壳低聚糖具有比壳聚糖更好的溶解性和生理活性,采用酶法降解具有反应条件易于控制、产物安全性高和环境污染少等独特的优越性,因此,筛选壳聚糖降解酶的方法和条件有重要意义。对壳聚糖酶生产菌所产壳聚糖酶的培养条件进行了初步研究,并对测定壳聚糖酶酶活力的DNS法进行了研究。结果表明,DNS法的最大吸收波长在495nm。该实验所用菌种产壳聚糖酶的培养条件以培养时间为60h,初始pH值为5.0,装液量为50mL比较适宜。

Chitosan is a high polymer and a cation exists only in nature, which can be biologically degraded and applied in the field of agriculture, medicine and food, etc. Low weight molecular of chitosan has better solubility and physiological functions than chitosan. The enzymic hydrolysis has some advantages of easy-controllable reaction, high security of products and less environmental pollution. Thus, the method and conditions for enzymic hydrolysis of chitosn are important. This paper deals with the incubation conditions for chitosanase producing microorganism basically and DNS method for measuring enzyme activity of chitosanase. The results showed that the maximum absorption wavelength of DNS method was 495 nm. The optimum incubation conditions for producing chitosanase were 60 h of the incubation time, 4.5 of the initial pH value and 50 mL of medium volume.