重组干扰素γ及肿瘤坏死因子增强巨噬细胞产生超氧阴离子的初步研究

A Preliminary Study on Increased Superoxide Anion Release from Murine Macrophages in Response to Recombinant Human Interferon-γ and Tumour Necrosis Factor

本文报道重组人干扰素(IFN-γ),肿瘤坏死因子(TNF-α)及细菌内毒素(LPS)刺激小鼠巨噬细胞,激发其产生超氧阴离子的能力。结果表明这两种细胞因子均可直接激发超氧阴离子的产生并呈剂量-时间依赖性,但内毒素无此作用。当肿瘤坏死因子或干扰素γ预激24h后再次激发可增强超氧阴离子产生,提示在炎症过程中,细胞因子诱导产生的超氧阴离子可能对胞内寄居病原菌及肿瘤细胞杀伤中起重要的作用。

Superoxide anion releasing from activated murine macrophages stimulated with recombinant human interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α) and bacterial lipopolysaccharidc were studied. The results showed that both of the cytokines enhanced superoxide anion release in a dose and time - dependent manner, but lipopolysaccharide had no such an effect as compared with IFN-γ and TNF-α. It was also shown that O2- generation from macrophages was highly enhanced after primed with IFN-γ or TNF-α for 24 hours. It was concluded from these data that cytokines released from macrophages and lymphocytes during inflammatory reactions could promote O2- generation which may play a crucial role in destruction or kill of intracellular pathogenic bacteria, and tumour cells.