应用固相微萃取技术分析番茄与天竺葵活体植株的挥发物

Analysis of volatile chemicals emitted from living tomato and geranium samples with SPME technique

应用固相微萃取技术 (SPME)和自行设计的挥发物收集装置对番茄和天竺葵的活体植株所释放的挥发性有机物 (BVOCs)进行了色谱分析。鉴定出番茄植株挥发物主要组成物质为α 蒎烯、α 萜品烯、水芹烯和桧烯等 ,天竺葵挥发物主要组成物质为α 蒎烯、莰烯、β 蒎烯、β 香叶烯、苎烯、石竹烯和α 草烯等。而作为对照的常规通气吸附法则由于背景噪声复杂而未能有效鉴别出这两种植物活体植株的挥发物组成。实验表明 ,本研究中建立的技术和装置能直接检测芳香植物活体所释放的挥发物的成分 ,并具有操作简便 ,无有机溶剂 ,重复性好等优点 。

Solid phase microextraction(SPME) technique was applied to collect the biogenic volatile organic compounds(BVOCs) emitted from living samples of tomato and geranium.A set of collecting devices designed by the authors was used in these experiments too.And then,the BVOCs collected were sent to be analyzed by the chromatogram analysis system.The major components in the BVOCs from tomato samples were identified as α pinene,α terpinene,phellandrene,sabinene and some unknown chemicals.And α pinene,camphene,β pinene,β myrcene,limonene,caryophyllene and α humulene were found in the BVOCs from geranium samples.In contrast,the control experiments which employed the conventional aerating absorption system could not tell the certain volatile chemicals of tomato and geranium because of the complex background.These experiments indicated that the technique and devices established in this research were effective to identify the BVOCs emitted from the living aromatic plants directly,with the advantages such as clean background,convenience,good repeatability and solvent free.So this system can be a valuable method to study the volatile second metabolic chemicals in living plants.