肝间质成分层粘连蛋白和透明质酸的研究及临床应用

Extracellular Matrix in Liver: Experimental Study and Clinical Application

作者对肝脏内的细胞外间质成分层粘连蛋白(Lammin,LN)和透明质酸(Hyaluronic acid,HA)进行了细胞学研究和病人血清含量检测,发现LN和HA可明显抑制人胎肝细胞的增值和脯氨酸合成的参入,而在慢性肝病尤其是肝硬变病人血清中的含量明显高于正常人,并且和肝功能损害程度呈正相关。LN和HA联合诊断肝硬变的符合率为90％。提示LN、HA在肝纤维化发生机制中可能起抑制作用,而在血清学方面可以作为判断慢性肝病损伤程度和诊断肝纤维化的指标。

Laminin(LN) and hyaluronie acid (HA) are extracellular matrix in liver. The effects of LN and HA on human fetal liver cells proliferation and collagen synthesis were observed and serum levels of LN and HA were assayed m patients with liver diseases by an enzyme immunoassay method. Both LN and HA had a significant effect on the inhibition of cell proliferation and collagen production. However, serum levels of LN and HA were significantly increased in liver cirrhosis comparing with healthy controls and with patients with non-liver diseases. The results suggest that LN and HA may play a role in the feed-back inhibition mechanism in liver fibrogencsis, and assay of LN and HA in serum may be useful to the diagnosis of liver fibrosis.