抗肺癌单抗3C_9识别的抗原检测及其血清诊断价值初步探讨

Assay of antigens with anti-lung cancer McAb 3C_9 and preliminary evaluation of serological diagnosis for lung cancer

采用单克隆抗体BA—ELISA结合抑制法,检测了血清中抗肺癌单抗3C_9识别的抗原(3C_9Ag),共检测肺癌50例,健康成人40例,肺部非肿瘤疾病21例。同时用放免法测定血清CEA。研究发现:3C_gAg对肺癌诊断敏感性为76％,特异性90.5％,准确性为80.3％;在NSCLC具有较高阳性率,其明显升高见于Ⅱ、Ⅲ期肺癌病人。与CEA相比,3C_9Ag在诊断肺癌的敏感性、准确性方面更为优越;二者联合检测可提高肺癌检出率。

The antigens(3C9Ag)which could be recognized by the anti-lung cancer monoclonal antibody 3Ca were assayed with BA-ELISA immunobinding inhibition test in 50 lung cancer patients,21 patients with non-cancerous pulmonary diseases and 40 normal subjects.Meanwhile radioimmunoassay was used to determine serum CEA.It was found that the sensitivity,specificity and accuracy for 3C9Ag to diagnose lung cancer were 76%,90.5% and 80.3% respectively.Much higher positive rates could be found in NSCLC patients and in those with stage Ⅱ or Ⅲ cancer.3CsAg was superior to CEA in its sensitivity and accurracy.Combined assay with 3C9Ag and CEA could increase the positive rate.