从南方山荷叶分离出一株产鬼臼毒素类似物的内生真菌

An Endophytic Fungus Producing a Substance Analogous to Podophyllotoxin Isolated from Diphylleia sinensis

从南方山荷叶 (Diphylleiasinensis)的根、根状茎及叶柄部位共分离出 5 4株内生真菌。通过薄层层析(TLC)对 5 4株内生真菌的液体发酵培养物进行了初步分析 ,再采用高效液相色谱 (HPLC)做进一步检测 ,得到 1株产鬼臼毒素类似物的内生真菌 (SJ2 1 ) ,经鉴定为纠错青霉 (Penicilliumimplicatum)。

54fungal isolates were isolated from the roots,rhizomes and petioles of Diphylleia sinensis which grew in Zhongdian county, Yunnan province. In the light of the test of fungi metabolites by TLC and HPLC, one isolate (SJ21) of the endophytic fungi, which could produce a substance analogous to podophylltoxin, was selected. SJ21 is Penicillium implicatum through identification.