登革Ⅱ型病毒经白纹伊蚊滞育卵的传递

Transmission of dengue-2 virus by diapausing eggs of Aedes albopictus

采用C6 36细胞培养分离病毒的方法检测感染登革Ⅱ型病毒的白纹伊蚊Aedesalbopictus滞育卵孵化的F1 代蚊虫感染率 ,从第一个生殖营养周期子代蚊虫中未分离到病毒 ,第二与第三生殖营养周期子代蚊虫最低感染率没有显著性差异 (χ2 =0 0 1,P >0 0 5 ) ,感染子代的批阳性率为 9.1% ,最低感染率为 1∶330 ;间接免疫荧光检测结果表明感染登革Ⅱ型病毒的白纹伊蚊滞育卵孵化的子代成蚊能通过叮咬将登革病毒传播给敏感乳鼠。这些研究结果表明登革病毒能在媒介滞育卵内存活并传至子代 。

Virus isolation in C6/36 cells was used for detection of the infection rates of F_1 progeny from diapausing eggs of infected Aedes albopictus. Eggs from three gonotrophic cycles (GC) were collected, and vertical transmission was not observed in the first gonotrophic cycle, while there was no significant difference observed between the infection rates of the GC2 progeny and that of the GC3 progeny (P>0.05). The total positive rate of pools was 9.1%, and the minimum infection rate was 1∶330. The results of indirect fluorescent antibody test showed that F_1 adult mosquitoes obtained from diapausing eggs can transmit dengue-2 virus to sensitive mice horizontally through biting. The horizontal transmission rate was 6.1%. These results indicate that dengue-2 can be transmitted vertically even the infected eggs are induced into diapausing, and F_1 adult mosquitoes raised from diapausing eggs can transmit dengue-2 virus to sensitive mice horizontally via biting.