两种鼠蚤在新羽化和吸血后不同时间三种酶的组织化学研究

Histochemistry of three enzymes in newly emerged and engorged adults of rat fleas Monopsyllus anisus (Rothschild) and Leptopsylla segnis (Schnherr)

采用组织化学技术 ,研究了不等单蚤Monopsyllusanisus (Rothschild)和缓慢细蚤Leptopsyllasegnis (Sch nherr)新羽化和吸血后 2 4h、4 8h和 72h碱性磷酸酶、酸性磷酸酶和三磷酸腺苷酶的分布和活性。显微摄影及定量图像分析结果显示 :新羽化蚤碱性和酸性磷酸酶主要存在于中肠、神经细胞核、精子束头部、精巢附腺、射精管、输卵管和受精囊附腺中 ;三磷酸腺苷酶各组织中均有分布。吸血消化后 ,两种蚤中肠 3种酶活性均有增强 ;除碱性磷酸酶在消化 72h后酶活性有所下降外 ,其余不同消化时间酶活性增强程度上不存在显著差异。两种蚤卵母细胞发育成熟过程中

The distribution and activity of alkaline phosphatase (AKP), acid phosphatase (ACP) and adenosine triphosphatase (ATPase) in newly emerged and engorged adults of Monopsyllus anisus (Rothschild) and Leptopsylla segnis (Schnherr) were studied with histochemistry techniques and image analysis of microscope photographs. The results indicated that in newly emerged adults, alkaline phosphatase (AKP) and acid phosphatase (ACP) were mainly distributed in midgut, nerve nucleus, testis, ejaculatory duct, oviduct and spermathecal gland, while adenosine triphosphatase (ATPase) was distributed in all tissues. After blood ingestion and digestion, the activity of three enzymes increased in midguts. In engorged adults, except that the activity of alkaline phosphatase (AKP) decreased 72 hours after blood ingestion, there was no significant difference in the increasing degree of the activity of the three enzymes in other time after blood ingestion. The activity of the three enzymes also increased during growing up of oocytes.