T细胞性NHL抗原受体基因重排分析

A study on antigen receptor gene rearrangements in 13 cases of T-cell non-Hodgkin's lymphoma

应用Southern blot和PCR技术对13例T细胞免疫表型的NHL进行基因重排分析。结果显示,11例(86％)肿瘤出现TCRβ基因克隆性重排,其中10例(91％)伴有TCRγ基因重排。提示基因重排分析是确立NHL克隆性和细胞源性的敏感而特异的标记。

The beta and gamma chain gene rearrangements of the T-cell receptors(TCR)were studied with Southern blot hybridization and polymerase chain reaction in 13 cases of non-Hodgkin's lymphoma with T-cell phenotype.It was found that 11 out of the 13 cases(86%)exhibited TCR beta gene rearragnement and 12 out of the 13(91%)displayed TCR gamma gene rearrangement.The findings suggest that gene rearrangements are more sensitive and specific markers to establish T-cell monoclonality and lineage and helpful to the morphological diagnosis and immunophenotyping for non-Hodgkin's lymphoma.